HIGH THROUGHPUT GENE EXPRESSION PROFILING

HIGH TROUGHPUT GENE EXPRESSION PROFILING STARTING FROM 6 SAMPLES

Flexible voucher option: Save your funds and budgets!

Buy now and perform this or any other project within 12 months.

Changes on project design possible at any time.

Service includes:

• mRNA isolation from total RNA starting from 6 samples
• NEBNext® Ultra™ II Directional RNA library preparation
• Quantification and QC of library
• Sequencing of 1 x 50 nt or 2 x 150 nt with Illumina NextSeq 2000™
• Data delivery of FASTQ files via download

• 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 168,75 €/sample, 48 samples*
• 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 173,75 €/sample, 36 samples*
• 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 178,75 €/sample, 24 samples*
• 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 188,75 €/sample, 12 samples*
• 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 193,75 €/sample, 6 samples*
• 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 232,50 €/sample, 48 samples*
• 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 237,50 €/sample, 36 samples*
• 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 242,50 €/sample, 24 samples*
• 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 252,50 €/sample, 12 samples*
• 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 257,50 €/sample, 6 samples*

¹ Depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

*Samples must be delivered as batch

• Bioinformatic data analysis 1: RNA-Seq Alignment to standar genomes and non-standard genomes² (STAR aligner, DRAGEN) from 40,- €/sample
• Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 or DRAGEN workflow from 40,- €/comparison
• Bioanalyzer™ check of RNA samples: starting from 10,- €/sample
• Qubit™ check with RNA assay: starting from 10,- €/sample
• rRNA depletion: starting from 80,- €/sample

²Available reference genomes include: H. sapiens hg19, H. sapiens GRCh38Decoy, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1