“All in One Microbiome” Package, 192 Samples

Description

High Throughput Amplicon Generation, Sequencing and Analysis for 192 Samples

 

Single Step Bacterial and Archaea microbiome analysis

Chose your option for Amplicon sequencing:

 

Bacterial microbiome analysis:

16S, V3-V4 region, Primer combination: 341F – 806R

16S, V4 region, Primer combination: 515F – 806bR

16S, V4-V5 region, Primer combination: 515F – 926R

 

Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2

 

Send us your gDNA samples and make use of our “All in One” Service:

 

• Quality control
• Single step amplicon generation with reduced bias
• Double indexing, quality check, quantification, normalization and pooling of amplicons
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output: 20-30 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Data delivery via FTP server