16S, ITS and 18S Microbiome Sequencing
High Throughput Amplicon Generation, Sequencing and Analysis
“All in One Microbiome” Packages: Single Step Bacterial and Archaea microbiome analysis (V3-V4, V4, V4-V5 and ITS1)
Package A: 288 samples only 26,52 € per sample
Package B: 192 samples only 31.54 € per sample
Package C: 96 samples only 47.08 € per sample
Package C: 48 samples only 76.17 € per sample
Bacterial/Archaea microbiome analysis:
16S, V1-V2 region, Primer combination: 27F – 338R
16S, V4 region, Primer combination: 515F – 806bR
16S, V4-V5 region, Primer combination: 515F – 909R
16S, V3-V4 region, Primer combination: 341f – 806R
Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2
2-Step microbiome analysis:
Eukaryotes (microbial) analysis
V9 region, Primer combination: Illumina_Euk_1391f – Illumina_EukBr_1510r, blocking primer optional
V8-V9 region, Primer combination: 18S-1422f – 18S_1797R
Bacterial and Archaea microbiome analysis
16S, V1-V2 region, Primer combination 27F – 338R
Fungal (18S) microbiome analysis:
ITS 1/2 region, Primer combination: ITS-u2 – ITS-p4
ITS, ITS 2 region, Primer combination: ITS3F – ITS4R
SSU region, SSUfungiF (5′-TGGAGGGCAAGTCTGGTG-3′) – SSUFungiR (5′-TCGGCATAGTTTATGGTTAAG-3′)
LSU region, LSU Fungi LR0R_f (5′-ACCCGCTGAACTTAAGC-3′) – LSU Fungi JH-LSU-369rc_r (5′-CTTCCCTTTCAACAATTTCAC-3′)
2-Step Barcoding
cytochrome b, cytb1 (5′-ccatccaacatctcagcatgatgaaa-3′) – cytb2 (5′-gcccctcagaatgatatttgtcctca-3′)
cytochrome oxidase I, CO I E (5′-ccagagattagagggaatcagtg-3′) – CO 1 F (5′-cctgcaggaggaggagaycc-3′)
2-Step Custom Amplicons:
Customer specific assays, design of custom primers