LIBRARY, SEQUENCING-RUN AND BIOINFORMATICS, 25 MIO PE

Description

LIBRARY, SEQUENCING-RUN AND BIOINFORMATICS

 

Services includes:

• Quality and quantity check of total RNA
• mRNA isolation from total RNA starting from 1 sample
• NEBNext UltraExpress Directional RNA library preparatio
• Quantification and QC of library
• Sequencing of 2 x 150 nt with Illumina NextSeq 2000
• Bioinformatic data analysis 1: 1: RNA-Seq Alignment to reference genomes* (STAR aligner, DRAGEN)
  
• Data delivery of FASTQ files via download

Output:

 

• 25 mio PE reads** (2 x 150 nt, 3.75 Gb)**

 

*Available reference genomes include:

H. sapiens hg19, H. sapiens hg38, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1

 

**depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%