Incredible 16S and ITS Microbiome Analysis Promotion
Prepaid option - order now and deliver your sample within the next 12 months:
“All in One Microbiome” Packages: High Throughput Amplicon Generation, Sequencing and Analysis
Package A: 48 samples only 75 € per sample
Package B: 96 samples only 50 € per sample
Package C: 192 samples only 32 € per sample
Chose your option of Amplicon sequencing:
Bacterial microbiome analysis:
16S, V4 region, Primer combination: 515F – 806R*
16S, V4-V5 region, Primer combination: 515F – 909R*
Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2**
Send us your gDNA samples and make use of our “All in One” Service:
• Quality control
• Single step amplicon generation with reduced bias
• Double indexing, quality check, quantification, normalization and pooling of amplicons
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output: 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Applications:
• Environmental Metagenomics (soil, water, air)
• Human or Animal Microbiome (skin, stool, blood, swab)
• Sterility Monitoring
• Detection of Contamination
• Monitoring of Biogas Plant
• Biosafety Monitoring
• Food Quality
• Clinical Samples
Sequencing of your ready to load 16S/ITS/18S libraries:
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Only 2600 €
You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material.
Primer sequence references:
*Apprill A, McNally S, Parsons R, Weber L. 2015. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75:129–137. Parada AE, Needham DM, Fuhrman JA. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 2016;18: 1403–1414.
**White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, NY. Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.
Simply order by e-mail: microbiome@starseq.com
You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material. Please contact us if you are interested in further service options or other gene regions and we will find YOUR optimal solution!
info@starseq.com
Prepaid option - order now and deliver your sample within the next 12 months:
“All in One Microbiome” Packages: High Throughput Amplicon Generation, Sequencing and Analysis
Package A: 48 samples only 75 € per sample
Package B: 96 samples only 50 € per sample
Package C: 192 samples only 32 € per sample
Chose your option of Amplicon sequencing:
Bacterial microbiome analysis:
16S, V4 region, Primer combination: 515F – 806R*
16S, V4-V5 region, Primer combination: 515F – 909R*
Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2**
Send us your gDNA samples and make use of our “All in One” Service:
• Quality control
• Single step amplicon generation with reduced bias
• Double indexing, quality check, quantification, normalization and pooling of amplicons
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output: 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Applications:
• Environmental Metagenomics (soil, water, air)
• Human or Animal Microbiome (skin, stool, blood, swab)
• Sterility Monitoring
• Detection of Contamination
• Monitoring of Biogas Plant
• Biosafety Monitoring
• Food Quality
• Clinical Samples
Sequencing of your ready to load 16S/ITS/18S libraries:
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Only 2600 €
You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material.
Primer sequence references:
*Apprill A, McNally S, Parsons R, Weber L. 2015. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75:129–137. Parada AE, Needham DM, Fuhrman JA. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 2016;18: 1403–1414.
**White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, NY. Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.
Simply order by e-mail: microbiome@starseq.com
You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material. Please contact us if you are interested in further service options or other gene regions and we will find YOUR optimal solution!
info@starseq.com
• Sequencing with Illumina MiSeq
• Fast turnaround time - daily runs. From DNA to data in 5,5 - 55 hours!
• highly multiplexed PCR amplicon sequencing
• ultra deep sequencing of tumor samples
• targeted resequencing
• hybrid capture
• ChIP-Seq
• small RNA sequencing
• RNA-Seq (microbial)
• sequencing and de novo assembly of small genomes, plasmids (BAC’s)
• metagenomics (16S etc.)
• library QC
• custom enrichment
• exome enrichment
• cancer panel
• up to 850 Mb and 12-15 M reads in 5,5 h (1 x 50 bp read length) Standard Flow Cell Reagentv2
• up to 3,8 Gb and 22-25/44-50 M reads in 24 h (1 x 150/2x75 bp read length) Standard Flow Cell Reagentv3
• up to 5,1 Gb and 24-30 M reads in 24 h (2 x 150 bp read length) Standard Flow Cell Reagentv2
• up to 300 Mb and 1 M reads in 24 h (2 x 150 bp read length) Nano Kit v2
• up to 1,2 Gb and 4 M reads in 24 h (2 x 150 bp read length) Micro Kit v2
• up to 8,5 Gb and 24-30 M reads in 39 h (2 x 250 bp read length) Standard Flow Cell Reagentv2
• up to 500 Mb and 1 M reads in 39 h (2 x 250 bp read length) Nano Kit v2
• up to 15 Gb and 44-50 M reads in 55 h (2 x 300 bp read length) Standard Flow Cell Reagentv3
Illumina Library Preparation for NextSeq™, HiSeq™ and MiSeq™ Sequencing:
single- and paired-end libraries from:
• genomic DNA
• ChIPseq samples
• plasmids
• cDNA
special libraries:
• metagenomic amplicon generation (V3-V4;V4; V4-V5; ITS; 18S)
• mate-pair library preparation
• enrichment of human exome or custom regions
• small RNA library preparation
RNA library preparation:
• total RNA
• mRNA
In vitro transposition based Illumina libraries (Nextera™ technology) from:
• only 1 ng DNA (small genomes, plasmids, amplicons)
• 50 ng DNA (large genomes)
Further options:
• Optimized and sharp size selection possible (SPRIselect™)
• Up to 96 indices possible
Get your personal quotation: info@starseq.com