Incredible RNA Sequencing Promotion

Prepaid option available - order now and deliver your sample within the next 12 months:

LIBRARY, SEQUENCING-RUN AND BIOINFORMATICS

Service includes:
• mRNA isolation from total RNA
• TruSeq stranded RNA library preparation
• Quantification and QC of library
• Bioinformatic data analysis: RNA-Seq Alignment to reference genomes* (TopHat, FPKM/RPKM, Variants, Fusion)
• Sequencing of 1 x 75 / 2 x 150 nt with Illumina NextSeq 500™
• Data delivery of FASTQ files via download

Output:
• 50 mio single-end reads** (1 x 75 nt, 3.75 Gb) for only 750,- € / sample
• 25 mio paired-end reads** (2 x 150 nt, 3.75 Gb) for only 790,- € / sample
• 50 mio paired-end reads** (2 x 150 nt, 7.5 Gb) for only 990,- € / sample

Price valid from the very first sample!


HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6 AND 12 SAMPLES

Service includes:
• mRNA isolation from total RNA for 6 or 12 samples
• TruSeq stranded RNA library preparation
• Quantification and QC of library
• Sequencing of 2 x 150 nt with Illumina NextSeq 500TM
• Data delivery of FASTQ files via download

Output:
• 25 mio paired-end reads** (2 x 150 nt, 3.75 Gb) for only 450,- € / sample, 12 samples
• 25 mio paired-end reads** (2 x 150 nt, 3.75 Gb) for only 550,- € / sample, 6 samples
• 50 mio paired-end reads** (2 x 150 nt, 7.5 Gb) for only 650,- € / sample, 12 samples
• 50 mio paired-end reads** (2 x 150 nt, 7.5 Gb) for only 750,- € / sample, 6 samples

Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes[s_1] (TopHat, FPKM/RPKM, Variants, Fusion) 80,- € / sample
Bioinformatic data analysis 2: Pairwise comparison of RNA data [s_1] to identify differentially regulated genes with Cufflinks workflow 175,- € / comparison
Bioanalyzer check of RNA samples: 40 € per sample

*Available reference genomes include: H. sapiens hg19, H. sapiens hg38, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1
**depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

Offer cannot be combined with other promotions or discounts. Valid until 31.12.2016
All prices without tax.

Simply order by e-mail: info@starseq.com

Illumina NextSeq 500 Sequencing Service
StarSEQ is the FIRST sequencing service provider with a NextSeq 500 in Europe.

Sequencing with Illumina's NextSeq 500 V2 chemistry - improved data quality and the highest yield of error-free reads!

• Ultra-Fast turnaround times. Data generation within 11 - 29 hours!
• Highly Multiplexed Sequencing
• Whole-Genome Sequencing
• Exome Sequencing
• Transcriptome-Sequencing
• ChIP-Seq
• small RNA sequencing
• Targeted Resequencing

Possible read length: 1x75 bp, 2x75 bp, 1x150 bp, 2x150 bp
You can order flexible quantities of reads: 25 Mio, 50 Mio, 75 Mio, 100 Mio, 130 Mio, 150 Mio, 200 Mio, 260 Mio, 400 Mio and 800 Mio!




Get your personal quotation: info@starseq.com


Illumina Library Preparation for NextSeq™, HiSeq™ and MiSeq™ Sequencing:
single- and paired-end libraries from:
• genomic DNA
• ChIPseq samples
• plasmids
• cDNA
special libraries:
• metagenomic amplicon generation (V3-V4; V4; V4-V5; ITS, 18S)
• mate-pair library preparation
• enrichment of human exome or custom regions
• small RNA library preparation
RNA library preparation:
• total RNA
• mRNA
In vitro transposition based Illumina libraries (Nextera™ technology) from:
• only 1 ng DNA (small genomes, plasmids, amplicons)
• 50 ng DNA (large genomes)
Further options:
• Optimized and sharp size selection possible (SPRIselect™)
• Up to 96 indices possible


Get your personal quotation: info@starseq.com